Storage

• 1．The kit can be transported at room temperature.
• 2．All reagents are stored at 2-8°C.
• 3．All reagents, when stored properly, are stable for 18 months from the time of delivery.

Apparatus and materials to be prepared by the user

*  Sterile 1.5ml micro centrifuge tubes               * 10µl/100µl/1000µl tips

*  Microcentrifuge capable of 14,000 × g            * Vortex mixer

Important notes

• 1. The RNase A solution should be all added into the Resuspension Buffer before use, mix and store at 2-8℃.
• 2. If the Lysis Buffer precipitated, it should be dissolved by warming to 37°C.
• 3. Please close the lid immediately after using Lysis Buffer so as to avoid acidification.
• 4. The kit can extract high-quality plasmid DNA from 1-5ml E.coli overnight cultured.