This kit is a column-type total RNA extraction kit based on the improved lysis and binding reagent components, which improves the lysis and binding performance of the reagent. The addition of chloroform realizes the separation of impurities such as RNA from DNA and protein, and optimizes the column membrane to improve its ability to adsorb RNA. After multiple washings, impurities are effectively removed to the greatest extent, and high-purity and high-quality total RNA is obtained. Nucleic acid can be extracted from a wide range of samples such as blood, animal and plant tissues, various microorganisms and cultured cells. High-quality nucleic acid products can be directly used in downstream molecular experiments such as Northern Blot, RT-qPCR, and NGS.