Storage and transportation

• The kit should be stored dry at room temperature（15～25℃）, The kit can be stored for up to 18 months if all components are kept properly.
• The kit can be transported at room temperature.

Technical Information

Apparatus and Materials to Be Supplied by the User

* Sterile 1.5 or 2.0 ml microcentrifuge tubes        * 10µl/100µl/1000µl tips

*  Absolute ethanol    * Microcentrifuge capable of 14,000g * Vortex mixer   * Water bath or cooling & heating block

Preparation

1. This product cannot be used with PAGE with urea.
2. Add ethanol as per the volume marked on label of bottle into Wash Buffer and then mix well.
3. If the gel slice is very big, please increase the dosage of PE Buffer properly but on more than 200μl, thus to guarantee that the gel slice is immerged completely.
4. The suitable volume of elution buffer for the kit is 50µl. The dosage of elution buffer can be adjusted according to practical experimental situation.
5. Please wash the fixed gel by water or 10mM Tris-HCl (pH7.0) for 2 or 3 times if the gel is fixed by acidic reagent such as acetic acid.