This kit allows the high efficient binding of DNA to our spin matrix while proteins and other impurities are removed by wash buffer. Nucleic acids are easily eluted with sterile water or Elution Buffer. This kit is designed for fast and efficient purification of plasmid DNA from 150 to 200 ml of E. coli culture. The maxi column has a plasmid DNA binding capacity of 1 mg.
The purified plasmid DNA is ready for downstream applications such as transfection of robust cells such as HEK293, restriction mapping, library screening, sequencing, as well as gene therapy and genetic vaccinations.
Storage and transportation
- The kit has demonstrated stability of 18 months when the RNase A should be stored at 2-8℃,others at room temperature.
- The kit can be transported at room temperature.
- Resuspension Buffer should be stored at 2°C-8℃ once RNase A is added.
Apparatus and materials to be prepared by the user
* 50ml centrifuge tubes * 10µl/100µl/1000µl tips
* Centrifuge capable of 14,000g * Absolute alcohol
Important note
1）Spin down RNase A vial briefly. Add the RNase A solution to Resuspension Buffer and mix well before use. Resuspension Buffer should be stored at 2°C-8℃ once RNase A is added.
2）Wash Buffer: Add the alcohol as the volume marked on bottle label and mix well.
3）Lysis Buffer precipitates below room temperature. It is critical to warm up the buffer at 50°C to dissolve the precipitates before use.
4）Neutralization Buffer may form precipitates below 10°C, warm up at 37°C to dissolve the precipitates before use.
5）Carry out all centrifugations at room temperature.
6) Add 2ml Balance Buffer to each MaxiSpin column, incubate at room temperature for 2 mins,centrifuge at 5,000 x g for 3 min. Discard flow-through. It can improve the yield of DNA significantly.
İnkilap Mah.Küçüksu Cad.No: 107 Kat:2 Daire:8 Ümraniye / İstanbul / Turkey