BioFast Biozol Reagent is a ready-to-use reagent for the isolation of total RNA from animal or plant tissue. The operation of the kit is very simple and easy. First, add the sample to the grind tube with Biozol for complete lysis. Second, add chloroform to the solution and then centrifuge the grind tube. The homogenate will be separated to three phases: upper aqueous phase, interphase phase and organic phase. RNA remains exclusively in the aqueous phase; the RNA is recovered by precipitation with isopropyl alcohol.
Since the operation of BioFast Biozol is very easy, it can realize the extraction for several samples simultaneously. And total RNA isolated by BioFast BIOZOL Reagent is free of protein and DNA contamination. It can be used for Northern blot analysis, dot blot hybridization, poly (A)+ selection, in vitro translation, RNase protection assay, RT-PCR assay, cDNA library building and other RNA research.
RNase can be introduced accidentally into the RNA preparation at any point in the isolation procedure through improper technique. Because RNase activity is difficult to inhibit, it is essential to prevent its contamination. The following guidelines should be observed when working with RNA.
İnkilap Mah.Küçüksu Cad.No: 107 Kat:2 Daire:8 Ümraniye / İstanbul / Turkey