BioEasy Taqman PCR Magic Mix

This product is a standard reagent that is designed for the technology of Taqman probe. User can do real-time PCR reaction directly by adding primer, template and Taqman probe.

 

Applied Instrument

Line-Gene series Real-time PCR system from BIOER TECHNOLOGY CO., LTD. or the same type of instrument from other companies.

Features

  • 1. Template

This kit applies to plasmid DNA(10-107 copies), genomic DNA (100pg-1ug)or cDNA(1pg-100ng). To get the best result, the length of expansion segment should be in the bound of 50-200bp.

  • 2. Primer and Probe

Primer and probe are the important parameters in the Real-time PCR reaction. During the time of designing primer and probe, suggest using special software, such as Primer Express, beacon Designer, AlleledID. Normally, the concentration of primer and probe is adjusting during 0.1uM-1.0uM.

  •  Concentration of ion

The PCR reaction liquid in this kit contains Mg2+ in end concentration 3mM. This is suitable for normal situation. However, you can adjust the concentration of Mg2+ for different intention segment.

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