This kit applies to plasmid DNA(10-107 copies), genomic DNA (100pg-1ug)or cDNA(1pg-100ng). To get the best result, the length of expansion segment should be in the bound of 50-200bp.
Primer and probe are the important parameters in the Real-time PCR reaction. During the time of designing primer and probe, suggest using special software, such as Primer Express, beacon Designer, AlleledID. Normally, the concentration of primer and probe is adjusting during 0.1uM-1.0uM.
The PCR reaction liquid in this kit contains Mg2+ in end concentration 3mM. This is suitable for normal situation. However, you can adjust the concentration of Mg2+ for different intention segment.