Adopting nucleic acid amplification technique and combining intermediate fluorescence method, SYBR Green I real-time PCR can do detection and quantification on target gene fast and properly. The kit offers 2×SYBR Mix, including all components except for Taq DNA polymerase and primers. During operation, user can do real-time PCR reaction directly by adding primer, template and Taq DNA polymerase, which is very simple.

SYBR Green I is one kind of fluorescence dye, which will excited fluorescence signal after specially inserted in dsDNA molecule. In PCR reaction system, when SYBR Green I dye combine with dsDNA, and excited with excited light, via testing strength of fluorescence, user can calculate the quantity of dsDNA. The advantage of fluorescence dye is that: it can be used in testing any dsDNA amplification, without design of probe, and make the testing method easier, meanwhile, it can decrease cost, what’s more, user can analyze melt curve directly. As soon as the PCR test finish. By the analysis of melt curve, user can judge whether there are existing aberrance or non-specific amplification. The Max Absorb wave length of SYBR Green I is 497nm, the Max. Emission wavelength is about 520nm.

Instrument

Bioer Line-Gene Real Time PCR Detection System or the same kind of the instruments in other companies.