The kit provides a very simple, fast and effective technique for the isolation of pure high– molecular-weight genomic DNA in Oral swabs. Based on the remarkable selectivity on genome DNA of Biospin membrane, the simple purification procedure involves only a few steps and allows isolation of high yields of pure genomic DNA within 30 minutes. No any expensive equipments are required, using of  toxic or  hazardous  reagents,  such  as  phenol  or  chloroform  is  completely  avoided.  In  general,   0.5～3.5μg genomic DNA can be acquired using the Kit. The pure DNA can be applied extensively in PCR/Real-time PCR、sequencing、Southern blot、mutant analysis、SNP, and so on.