MagaBio plus Total RNA Purification Kit

The kit provides a very simple, fast and cost effective technique to isolate high quality RNA. Using one simple protocol, high yield of purified RNA can be isolated from various sources including blood, culture cells, leukocytes, tissue and plant. MagaBio sample processing is based on proprietary magnetizable particles–MagaBio. The pure RNA can be applied extensively in RT-PCR, Northern hybridization, mutant analysis, RNAi and the others.

According to the special interaction, use MagaBio nucleic acid separation system with a general protocol—sample processing, MagaBio adsorption, washing and elution, and can go high-throughput.

Features

Storage

 

  • 1．The kit can be transported at room temperature.
  • 2．All reagents are stored at room temperature.
  • 3．All reagents, when stored properly, are stable for 12 months from the time of delivery.

 

Advantage

MagaBio Magnetic technical have great advantages:

  • 1. Mini sample, high purification
  • 2. Simple and streamline separation procedure, used for auto-platform
  • 3. First elution can acquire 85% or more
  • 4. No high salt solution. no inhibitor
  • 5. No spin column

Apparatus and materials to be prepared by the user

 

  • 1．Magnetic Rack or Bioer NPA-32+ purification instrument
  • 2．Water bath or Dry bath
  • 3．Vortex mixer
  • 4．Absolute alcohol

 

Important Notes
  • 1. Add the alcohol (as the volume marked on bottle label) to the DNase Stop Buffer and Wash Buffer, and then mix them well.
  • 2.  MagaBio Reagent should be mixed well (particles are uniformly suspended) every time.
  • 3. MagaBio plus total RNA Purification Kit has been optimized for use with fresh whole blood and leukocytes, frozen blood samples cannot be used.
  • 4. Proper microbiological, asceptic technique should always be followed when working with RNA. All plasticware and glassware should be free of RNase contamination. Keep the tubes closed during the procedure, work quickly, and keep the purified RNA on ice until used.
  • 5.  If the room temperature is too low ,we can put the ML Buffer at 45℃ warm bath for preheating.

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