RT PCR is a useful technique for the investigation of gene expression, viral load, pathogen detection, and numerous other applications. When analyzing gene expression or viral load, the RNA of interest first needs to be reverse transcribed into cDNA. The subsequent PCR can be performed separately (two-step RT-PCR).

BioRT Two Step RT-PCR Kit is designed for two step RT-PCR of RNA samples from various  sources.The kit includes all the necessary reagents for cDNA synthesis and subsequent PCR. Either total RNA, messenger RNA, viral RNA or in vitro transcribed RNA can be used as a template for reverse transcription. The kit includes both random primers and oligo(dT18) primers. The user can choose either of these or alternatively use gene specific primers. The  reverse transcriptase in  the BioRT Two  Step RT-PCR  Kit is AMV,  which provides up to 60℃ RT temperature and provides higher sensitivity and higher yield to cDNA’s synthesis and PCR. The performance of the PCR step is based on a high fidelity Taq mix DNA polymerase. The kits can synthesis up to 12kb cDNA and 6kb DNA. The reaction buffer is optimized for a kind of reaction system (10ul for RT and 25ul for PCR).