RT-PCR is a technology which combines reverse transcription (RT)  and polymerase chain reaction (PCR) to detect target RNA. RT-PCR technology can be used to detect gene expression in cells and tissues, cDNA sequences of cloned specific genes and RNA viruses.
Different from two steps RT-PCR in which RT and PCR separated in two tubes, BioRT One Step RT-PCR Kit is designed to combine RT and PCR in one tube, and no additional reagents are needed during the process. High-quality AMV reverse transcriptase produced in USA and Hot start Taq polymerase are included in this kit, and an optimized buffer ensure the best performance of the two enzymes. AMV reverse transcriptase that produce high-yield cDNA and highly purified recombinant Taq polymerase with monoclonal antibody used together can get up to 4kb products.
BioRT One Step RT-PCR Kit adopts one tube system, and this system can reduce the potential for contaminating the samples and provide specific, sensitive and convenient analysis of RNA.