BioRT cDNA First Strand Synthesis Kit provides the materials needed to rapidly and reliably synthesis first strand cDNA from RNA. It uses AMV reverse transcriptase to synthesize first strand cDNA from an RNA population.
The kit includes all the necessary reagents for first strand cDNA synthesis. The kit includes both random primers and oligo(dT18) primers. The user can choose either of these or alternatively use gene specific primers. The reverse transcriptase in the BioRT cDNA First Strand Synthesis Kit is AMV, which provides up to 60℃ reverse transcriptase temperature and provides higher sensitivity and higher yield to cDNA’s synthesis and PCR. The kits can synthesis up to 10kb cDNA. The reaction buffer is optimized for a kind of reaction system.
3. AMV reverse transcriptase, Taq polymerase and RNase inhibitor should be slowly pipetted after centrifuging, and put back at -20℃ later;
4. Avoid frequently freezing and thawing dNTP;
5. Specific primers should be used and concentration of primers should be optimized and We suggest 0.4μM as a starting point for optimizing; Oligo-dT and Random primers are not suitable for this kit;
İnkilap Mah.Küçüksu Cad.No: 107 Kat:2 Daire:8 Ümraniye / İstanbul / Turkey