Adopting nucleic acid amplification technique and combining intermediate fluorescence method, SYBR Green I real-time PCR can do detection and quantification on target gene fast and properly.

The kit offers 2×SYBR Green Mix, including Low ROX and other components except for template DNA and primers. During operation, user can do real time PCR reaction directly by adding primers and template DNA,which is very simple. The kit has the advantage of high efficiency in amplification, specificity, good repeatability, and high sensitivity and so on after the improvement of the reaction solution and the application of higher quality enzymes, and it’s high GC sequence reaction and quantify is greatly increased.

Instrument

The reagent can be used in series Real Time PCR detection system of Bioer or the same kinds of the instruments of other companies; especially suitable for Applied Biosystems 7500, 7500 Fast, ViiA™7; Stratagene MX4000™, MX3005P™, MX3000P™, QuantiStudio™ 12K Flex .